Studies on mosquito natural infection with Japanese encephalitis virus in border area,Yunnan Province

Objective:To study the mosquito natural infection with Japanese encephalitis (JE) virus and evaluation on their vector status.Methods:Collecting mosquitoes from the indoor and the stables of livestocks in villages in the night,and the outdoor bamboo forests in the daytime.Using C6/36 or BHK 21 cells and suckling mice to isolate viruses.Results:92 748 adult female mosquitoes belonging to 45 species of 6 genera were captured.Forty-two strains of JE virus were isolated from 13 species of mosquito,of those,15 strains were isolated from Culex tritaeniorhynchus,5 strains from Cx.whitmorei,4 strains from Cx.pseudovishnui,2 strains from Cx.fuscocephalus,4 strains from Anopheles sinensis,3 strains from Aedes vexans,2 strains from Ae.albopictus,2 strains from Ae.lineatopennis,a strain from Cx.gelidus,a strain from Cx.annulus,a strain from An.vagus,a strain from Ae.assamensis,a strain from Mansonia uniformis.Conclusions:Cx.tritaeniorhynchus might be the principal transmitting vector of JE in Border area, Yunnan.Cx.whitmorei,Cx.pseudovishoui,Ae.vexans and Ae.albopictus are also considered to be transmitting vector of JE in Yunnan.So Culex are regarded as the main vector of JE in night-actived mosquitoes and Aedes are regarded as the main vector of JE in daytime-actived mosquitoes in the area.