Investigation of differential gene expression profiles in mice cryptorchid testes by cDNA microarray

AIM:To study the differential gene expression profiles between the normal and cryptorchid mice testes by cDNA microarray. METHODS: BLAC mice were unilaterally produced cryptorchidism artificially. Four weeks later, we extracted mRNA from both normal and cryptorchid testes, produced cDNA probes and employed them on cDNA microarray to detect the differential gene expression profiles. RNA dot blotting was taken on COX8a, a down-expressed gene in the previous hybridization, to validate the microarrays. RESULTS: Thirty-four differential expressed genes related to cryptorchism were found. CONCLUSION: Screening of the differential gene expression profiles between the normal and cryptorchid mice testes by cDNA microarray can be used in the study of cryptorchid-related genes and further researches. COX8a may play a role in the development and progression of cryptorchidism.