Cloning and expression of hTERT~(1658-2070) in E. Coli

Objective To experss a fragment of human telomerase reverse transcriptase (hTERT) with special function in E. Coli by genetic engineering. Methods A fragment of hTERT cDNA 1658 2070 was amplified by PCR method and was cloned into expression plasmid. Then the recombinant plasmid was transformed into E. Coli strain TG1 and the expression strain was obtained. DNA sequencing and Western blot was used to characterize the expressed protein. Results The experessed protein was GST hTERT fusion protein with the molecular mass of 38.8 kDa. It was confirmed by DNA sequencing and Western blot. Conclusion The fragment of hTERT cDNA was cloned and expressed in E.Coli.