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The Utilities of Fluorescence in Situ Hybridization Technique on R-banding Treated Slides

Liu Shi-he

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Abstract

Objective To investigate the utilities of fluorescence in situ hybridization technique(FISH) on R-banding treated slides.Methods FISH was performed with PML/RARa probes on R-banding treated slides of 17 untreated M3 patients.Results Five positive specimens and one negative specimens were revealed in six specimens of poor quality R-banding slides.Complex aberrant translocations were revealed in 2 specimens and were mapped.The PML/RARa fusion gene was revealed in 9 specimens with t(15;17) karyotype.Conclusion The R-banding with FISH techniques as compared with conventional karyotype analysis and direct FISH test in analysis of complex aberrant translocation.They have widly applied future for unrecognized chromosome abnormalities.

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What this paper is about

Objective To investigate the utilities of fluorescence in situ hybridization technique(FISH) on R-banding treated slides.Methods FISH was performed with PML/RARa probes on R-banding treated slides of 17 untreated M3 patients.Results Five positive specimens and one negative specimens were revealed in six specimens of poor quality R-banding slides.Complex aberrant translocations were revealed in 2 specimens and were mapped.The PML/RARa fusion gene was revealed in 9 specimens with t(15;17) karyotype.Conclusion The R-banding with FISH techniques as compared with conventional karyotype analysis and direct FISH test in analysis of complex aberrant translocation.They have widly applied future for unrecognized chromosome abnormalities.

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Available abstract

Objective To investigate the utilities of fluorescence in situ hybridization technique(FISH) on R-banding treated slides.Methods FISH was performed with PML/RARa probes on R-banding treated slides of 17 untreated M3 patients.Results Five positive specimens and one negative specimens were revealed in six specimens of poor quality R-banding slides.Complex aberrant translocations were revealed in 2 specimens and were mapped.The PML/RARa fusion gene was revealed in 9 specimens with t(15;17) karyotype.Conclusion The R-banding with FISH techniques as compared with conventional karyotype analysis and direct FISH test in analysis of complex aberrant translocation.They have widly applied future for unrecognized chromosome abnormalities.

Key concepts: Karyotype, Fluorescence in situ hybridization, Chromosomal translocation, Fish <Actinopterygii>, Biology, Chromosome, In situ, Cytogenetics

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