2008•Cytogenetics and Cell GeneticsRequires access

Fluorescent in situ hybridization on archival G-banded slides

Wael Ei-Rifai, Sakari Knuutila

Abstract

We report a simple procedure that allows the application of chromosomal in situ suppression hybridization to standard G-banded and Entellan-mounted archival preparations that had been stored for 4-8 y. The procedure includes removing the cover slips in xylene, refixation in buffered formaldehyde-acetone mixture, and washing in 2 x SSC, Tween 20.

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Available abstract

Key concepts: Biology, In situ, In situ hybridization, Formaldehyde, Fluorescence, Fluorescence in situ hybridization, Chromatography, Molecular biology