Determination of Adprin in chicken tissues by high performance liquid chromatography-tandem mass spectrometry

A high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) method was established for the determination of Adprin in chicken tissuses. The samples of muscle,liver,and kidney were extracted by methylenechloride,and the plasma samples were prepareed with C18solid-phase extraction column. The separation of Adprin was performed on Agilent 1100 system with Luna C18(2)column(5 μm,150 mm×2. 00 mm) and the elution solvent of methanol(containing 0. 1% fomic acid) and water(containing 0. 1% fomic acid) at a flow rate of 0. 2 mL·min-1. The electrospray was operated under the positive ionization mode and the drug was identified under multiple reaction monitoring(MRM) mode. The method was quantified by the extemal standard method. Results: the linear range was from 1 to 200 μg·kg-1(μg·L-1) with correlation coefficients(R2) more than 0. 999. The limits of detection(LOD) were 0. 3,0. 6,0. 3 and 0. 6 μg·kg-1(μg·L-1),and the limits of quantification(LOQ) were 1. 0,2. 0,1. 0 and 2. 0 μg·kg-1(μg·L-1) respectively in chicken tissuses. The average recoveries varied from 80. 3%-94. 9%. The intra-RSD and the inter-RSD were in the range of 2. 2%-7. 1%. Conclusion: The proposed method was simple,rapid,sensitive and suitable for the quantitative determination and confirmation of Adprin.