Deletion and mutation of p16 gene in primary gastric carcinoma

Objective:To study the relationship between the deletion and mutation of p16 gene in exon 2 and the occurrence of primary gastric carcinoma.Methods:Using molecular biological methods that include genome DNA extraction from fresh tissue and PCR-SSCP analysis technique,it was studied that the deletion and mutation of p16 gene in exon 2 in 30 cases of primary gastric carcinomas.Results:The total alteration rate of p16 gene in exon 2 in primary gastric carcinomas was 20.00%(6/30) in which the deletion rate was 10.00%(3/30) and the mutation rate was 10.00%(3/30).In contrast,neither deletions nor mutations was found in the control group of adjacent gastric mucosa,respectively with significant difference(P0.01).Conclusion:The deletion and mutation of p16 gene in exon 2 is related to the genesis of primary gastric carcinoma.