Efficacy of rhTNFR:Fc on ventricular arrhythmia in rats with acute myocardial infarction in vivo

Objective:To explore the efficacy of recombinant human tumor necosis factor-Fc fusion protein(rhTNFR:Fc)on ventricular arrhythmia induced by acute myocardial infarction in Rats in vivo.Method:Two hundred and forty rats randomized into sham operation group,acute myocardial infarction group and rhTNFR:Fc group.Anterior wall myocardial infarction was produced in AMI group by ligating the left anterior descending coronary artery,and it was not produced in sham operation group,while the rhTNFR:Fc group was treated with rhTNFR:Fc,a TNF-α sequestrant,24 hours before LAD coronary artery ligation.ECG was observed and ventricular arrhythmias induced by programmed electrical stimulation were recorded at baseline,10 min,20 min,30 min,60 min,3 h,6 h and 12 h after ligation;while expression of TNF-α among different groups was detected by immunohistochemistry.Result:Ventricular arrhythmia appeared by 10 min after ligation,reached the climax at 10-30 min,with a peak incidence at 15-25 min,and recovered gradually then.The expression of TNF-α was in the similar way.Compared with the AMI group,there was less incidence of ventricular arrhythmias and TNF-α in the rhTNFR:Fc group,P0.05.Conclusion:rhTNFR:Fc could markedly reduce the incidence of ventricular arrhythmia in rats with AMI in vivo.