Experimental Studies on the Toxicity of Dingchuan Granule

Objective To observe the acute toxicity of Dingchuan granule(DCG) in mice and the long-period toxicity in rats,respectively.Methods LD50 of DCG were tested in mice by Bliss methods.Rats were administered with repeated gastric infusion of DCG for 60 days and body weight,hematological and hematobiochemical parameters,coefficient and histomorphological figure of organs were examined in 60 days and 21 days after the cessation of DCG.Results The LD50 of KM mice was 171.09g/kg.In the long-period toxicity test,DCG was administered repeatedly for 60 days,the dose were 72.6,36.3,18.2 g/kg respectively(It refered to the dose of material,which amounted to 52.6,26.3,13.2 times of 50 kg human clinic use).There is no other abnormal changes about color patem,action,urine and stool,but cibation, hydroposia,body weight and index part of hematological and hematobiochemical parameters,coefficient and histomorphological figure of organs were examined during administration phase.The rats were examined in 21 days after the cessation of DCG,the abnormal changes of index was completely recovered.After administered repeatedly for 60 days and in 21 days after stopping the drug,there had no obvious abnormality for the important organ pathology examination.Conclusion The LD50 of mice is 171.09 g/kg.DCG administration by long-period large dose has a certain effect on rats,but the changes can completely recovery after drug withdrawal.