Effect of Different Culture Conditions on Mycelium Morphology and Activity of Exopolysaccharides from Ganoderma lucidum in Submerged Culture

Submerged fermentation is an important method to produce active metabolites of the edible and medicinal mushroom and attacted attentions of many researches. For obtaining polysaccharides of high yield and activity through regulating the fermentation conditions,effect of culture conditions(including culture time,initial p H and culture temperature) on G. lucidum mycelium morphology and biosynthesis and antitumor activity of G. lucidum exopolysaccharides were studied in this work. The G. lucidum pellets were divided to small,middle and large pellet by the diameter. The results indicated that the main form of mycelium was the M pellet,and the high percentage of M and S pellet was beneficial to the exopolysaccharides production. Moreover,high ratio of S to L pellet was good for the accumulation of G. lucidum exopolysaccharides. The result of antitumor activities of different G. lucidum EPS showed that higher inhibition effect on Hepa 1-6and MDA-MB-231 cells was obtained in culture condition of initial p H 4.0 and 30 ℃.