Stimulatory effect of 17β-estradiol on the proliferation of cultured HeLa cervical carcinoma cell line

Objectives:To study the effects of 17β-estradiol on the proliferation of human cervical carcinoma HeLa cells. Methods:Cell culture techniques, growth curves, MTT flow cytometry and laser scanning confocal microscope were used in the study. Results:17β-Estradiol(10 -6 mol/L) makes the curve move upward.17β-Estradiol(10 -8～10 -6 mol/L) significantly stimulated the proliferation of HeLa cells in a dose-dependent manner.17β-Estradiol(10 -6 mol/L) stimulated the proliferation of HeLa cells at G 1/S phases. It blocked G 1 phase from 70.0% to 55.5%, and stimulated S phase from 17.3% to 29.0%.17β-Estradiol (10 -6 mol/L) also increased the release of calcium in HeLa cells. Conclusions:17β-Estradiol can significantly stimulate the proliferation of cervical carcinoma cells possibly by activating cell cycle and stimulating the release of calcium in HeLa cells.