Experimental study on the relationship between cell apoptosis and the expression of the related cytokines in rats with duodenogastric reflux

Objective To study on the gastric mucosal apoptosis and the expression of cell cytokines caused by duodenogastric reflux(DGR),and investigate the possible pathogenesis of gastric mucosa injury in rats with DGR.Methods Ten adult SD rats were intubated duodenum to collect duodenal mixed juice(including bile,pancreatic juice plus duodenal juice).Another sixteen SD rats were randomly divided into two groups.The model group with DGR was transfused with duodenal mixed juice to stomach and the control group was transfused with 0.9% saline.The rats were executed after 14 days.Cell apoptosis in gastric mucosa was determined by TUNEL technique.Immunohistochemical staining was used to detect the expression of TNF-α,ET-1 and NOS-2 in gastric mucosa.Results The pathology of gastric mucosa demonstrated that rat models with DGR were successful.The apoptosis cells could be seen intensively in mucosa in DGR model group.Apoptosis index(AI) in DGR model group was significantly higher than that in control group(P0.05).The positive expression of TNF-α,ET-1 and NOS-2 in DGR model group were significantly higher than those in control group(P0.05).Conclusion Cell apoptosis and over expression of TNF-α,ET-1 and NOS-2 in gastric mucosa may be major pathogenesis of gastric mucosa injury and cell canceration with DGR.