ScholarLens Back to search
2012•Zhongguo yancao kexueRequires access

Construction of Tobacco Virus Resistance-related R Genes RNAi Expression Vectors

Yuanying Wang

Open publisher page 0 citations

Abstract

Gene silence mediated by RNA interference has been shown to be a highly rapid and effective method for breeding resistance to virus and analyzing plant functional genomics.Building of RNAi expression vector and being transformed into tobacco can obtain virus resistance tobacco materials and provide a new method for identification of tobacco functional genes in a large-scale.The partial sequence of R genes with specific banding site attB were amplified by PCR technology,primers were designed according to the sequences obtained from Nicotiana tomentosiformis sequencing.Using GATEWAY technology,the RNAi expression vector of R gene was constructed successfully by insered the partial sequence of R genes into the vector of pH7GWIWG2(I).

About this research paper

What this paper is about

Gene silence mediated by RNA interference has been shown to be a highly rapid and effective method for breeding resistance to virus and analyzing plant functional genomics.Building of RNAi expression vector and being transformed into tobacco can obtain virus resistance tobacco materials and provide a new method for identification of tobacco functional genes in a large-scale.The partial sequence of R genes with specific banding site attB were amplified by PCR technology,primers were designed according to the sequences obtained from Nicotiana tomentosiformis sequencing.Using GATEWAY technology,the RNAi expression vector of R gene was constructed successfully by insered the partial sequence of R genes into the vector of pH7GWIWG2(I).

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Gene silence mediated by RNA interference has been shown to be a highly rapid and effective method for breeding resistance to virus and analyzing plant functional genomics.Building of RNAi expression vector and being transformed into tobacco can obtain virus resistance tobacco materials and provide a new method for identification of tobacco functional genes in a large-scale.The partial sequence of R genes with specific banding site attB were amplified by PCR technology,primers were designed according to the sequences obtained from Nicotiana tomentosiformis sequencing.Using GATEWAY technology,the RNAi expression vector of R gene was constructed successfully by insered the partial sequence of R genes into the vector of pH7GWIWG2(I).

Key concepts: Gene, RNA interference, Biology, Functional genomics, Genetics, Tobacco mosaic virus, Vector (molecular biology), Expression vector

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Construction of Tobacco Virus Resistance-related R Genes RNAi Expression Vectors — Research Paper | ScholarLens