2009•Acta Agriculturae Boreali-SinicaRequires access

Cloning of Maize ZmPT6 Gene and Construction of RNAi Expression Vector

Fengbin Song

Abstract

The arbuscular mycorrhizal symbiotic phosphate transporter gene ZmPT6 was cloned from Zea mays Zhengdan 958 genomic DNA and RNA by PCR method.Sequences analysis showed that ZmPT6 full-length cDNA 1 665 bp,and contain a intron of 97 bp.ZmPT6 belong to member of Pht1 family,encoded 554 amino acid,and consist of 12 putative hydrophobic transmembrane domains.Three plant transformation vectors,sense vector p2300-35S-ZmPT6,antisense p2300-35S-ZmPT6r,and RNAi vector p2300-35S-ZmPT6RNAi were constructed and so as to provide basis of further genetic transformation and function of ZmPT6 gene.

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Key concepts: Gene, Complementary DNA, Genetics, Biology, Intron, Cloning (programming), Expression vector, Transformation (genetics)