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Detecting multidrug resistance gene expression by RT-PCR in children with acute leukemia

Fan Lihua

Abstract

Objective To study the relation between multidrug resistance gene mdrl and curative effects of chemotherapy and pro gnosis in children with acute leukemia.Methods mdrl mRNA expression in 33 children with acute leukemia was observed closely by semi-quantitative m ethod-RT-PCR,and the levels of mdrl were evaluated with the ratio of mdrl/β2M.Results The mRNA positive rate and ex pression levels of mdrl in refractory and relapse cases were significantly higher than those in first-visit and remission cases.Conclusion The expression lev els of mdrl mRNA might be related to refractory de gree and relapse in leukemia,which also has clinical guid ance significance in selecting chemotherapy methods and predicting prognosis.

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Key concepts: Refractory (planetary science), Acute leukemia, Chemotherapy, Leukemia, Multiple drug resistance, Messenger RNA, Gene, Clinical significance