Protective effect of Jiejufushenning on diabetic nephropathy in rats

Objective To investigate the effect of Jiejufushenning(JJFSN)on renal protection in diabetic nephropathy rats.Methods The diabetic SD rats induced by STZ were divided into 4 groups:model,JJFSN,irbesartan and JJFSN combined with irbesartan and control group.Rats in each group were respectively treated for 12 weeks by the appropriate intervention methods.The relative urinary albumin excretion rate(UAER),and other indicators were detected by routine analysis methods,and VEGF and BMP-7 in renal tissue were detected by immunohistochemical techniques,and PDGF-B in renal tissue was detected by Western blot,and ultramicrostructure of kidney was observed by transmission electron microscope.Results In addition to renal cortex BMP-7 expression of a significant reduction,the other indicators were significantly higher in diabetic rats.The ultramicrostructure of kidney was noticeably changed.While the above indexes in JJFSN,irbesartan and JJFSN combined with irbesartan groups were significantly better than in diabetic control group(P0.05),and ultramicrostructure of kidney changed was improved,and the improvement of all indexes in JJFSN combined with irbesartan group was most significant(P0.05).Conclusions Protective effect of JJFSN on diabetic nephropathy in rats is obvious.