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2006•Xibei nongye xuebaoRequires access

Effect of Darkness Pretreatment on Browning of Phalaenopsis Explants Cultured in vitro

Zhao Ling-li, Fan Chong-hui, Hong Ge

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Abstract

The variety R4 leaf of Phalaenopsis was used as explant,the effect of darkness pretreatment at different time on polyphenol oxidase(PPO) activity,total phenolic content and browning rate in virto were studied.The results as follows: Darkness pretreatment may decrease brown,especially of pretreatment for 10 d,browning rate was the lightest.The activity of PPO was the biggest when explant without darkness pretreatment.Total phenolic content didn't have accordant change rule,there was significant correlation between the browning rate and the activity of PPO in each treatment.

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What this paper is about

The variety R4 leaf of Phalaenopsis was used as explant,the effect of darkness pretreatment at different time on polyphenol oxidase(PPO) activity,total phenolic content and browning rate in virto were studied.The results as follows: Darkness pretreatment may decrease brown,especially of pretreatment for 10 d,browning rate was the lightest.The activity of PPO was the biggest when explant without darkness pretreatment.Total phenolic content didn't have accordant change rule,there was significant correlation between the browning rate and the activity of PPO in each treatment.

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Available abstract

The variety R4 leaf of Phalaenopsis was used as explant,the effect of darkness pretreatment at different time on polyphenol oxidase(PPO) activity,total phenolic content and browning rate in virto were studied.The results as follows: Darkness pretreatment may decrease brown,especially of pretreatment for 10 d,browning rate was the lightest.The activity of PPO was the biggest when explant without darkness pretreatment.Total phenolic content didn't have accordant change rule,there was significant correlation between the browning rate and the activity of PPO in each treatment.

Key concepts: Browning, Darkness, Polyphenol oxidase, Phalaenopsis, Explant culture, Botany, Catechol oxidase, Chemistry

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