Study on browning of Phalaenopsis amabilis in the tissue culture

The clustered shoots generated from proliferation period of Phalaenopsis amabilis were used as explant in order to study the browning of tissue culture.The treatments include two adsorbents: active carbon,PVP,and three pretreatments: explants preserved at 4℃ for 24 hours,with darkness pretreatment for 5 days and 10 days.The results indicated that active carbon was the best one of all the treatments,which could control browning and promote explant growth.Except for the explants preserved at 4℃ for 24 hours,the other treatments could decrease browning,but the effects were not as good as active carbon.