Method comparision of the kits from different manufactures in detecting of Leucine aminopeptidase

Objective To assess the comparability and bias of the LAP test results detected with leucine aminopeptidase(LAP) reagent from different manufacturers.Methods According to US National Clinical Laboratory Standards Committee(CLSI) EP9-A2,forty fresh serum samples covering different concentrations were continuously collected for 5 days.The collected samples were detected using LAP reagents made by 5 different manufacturers.Each sample was measured twice with the positive and negative order.The results were recorded to check the outliers and to calculate the linear equations and correlation coefficients,and to estimate the bias.Results The correlation between the tested reagents made by different manufacturers and Randox reagent was good(r0.975).Compared with Randox reagent,the expected bias of the results detected with the reagents manufactured by Meikang and Lideman was less than the allowable error region.Conclusion Though the correlation of the tested reagents is good,the detection results are not consistent.