Detection of soluble CD40L level in peripheral blood and bone marrow of patients with aplastic anemia

Objective To investigate the alteration of soluble CD40L level in patients with aplastic anemia(AA) and its possible role in the pathogenesis of AA, Methods Peripheral blood and bone marrow level of soluble CD40L in 40 AA patients and 20 normal controls was detected by enzyme linked immunosorbent assay (ELISA); Lymphocyte phenotype was analyzed by immunofluorescence and flow cytometry. Results The levels of sCD40L in peripheral blood and bone marrow both decreased signifieantly (P0. 001) ;the sCD40L level of AA was related to the count of peripheral WBC and platelet; the percentage of CD4+T cells decreased and the ratio of CD4/CD8 decreased. Conclusion The decreased level of sCD40L may be the potential reason of AA,detection of peripheral blood and bone marrow may be helpful for the diagnosis of AA and the application of CD40L will probably be an new method for the therapy of AA.