Study on Quality Standard of Mongolian Medicine Ganlekang Cpasules

OBJECTIVE:To establish the quality standard for Mongolian medicine Ganlekang capsule. METHODS:Lomatogonium rotatum,Gardenia jasminoides and Sophora flavescens were qualitative identified by TLC. The contents of matrine and oxymatrine were determined by HPLC;the determination was performed on lnertsil NH2(250 mm×4.6 mm,5 μm)column with mobile phase consisted of acetonitrile-3% phosphoric acid-absolute ethyl alcohol(80 ∶ 10 ∶ 10,V/V)at the flow rate of 0.1 ml/min. The column temperature was set at 30℃,and the detection wavelength was 220 nm. RESULTS:TLC spots of L. rotatum,G. jasminoides and S. flavescens were clear and well-separated. The linear range of matrine was 0.2-1.2 μg and that of oxymatrine was 0.3-1.8 μg.RSDs of precision,stability and reproducibility tests were all lower than 1.72%.The average recovery of matrine was 102.00%(RSD=0.93%,n=6)and that of oxymatrine was 98.02%(RSD%=0.78%,n=6). CONCLUSIONS:Established standard can be used for the quality control of Mongolian medicine Ganlekang capsule.