Formation of pseudomonas aeruginosabiofilm and antibiotic resistance

OBJECTIVE To establish the models of Pseudomonas aeruginosa,to compare the antibiotic susceptibility of P.aeruginosa in biofilms versus its counterparts in planctonic culture.METHODS Incubated isolates from clinic with the silicon catheters and observed the formation of biofilm on catheters by silver staining and scanning electron microscopy(SEM).Assay of determination the minimal inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) of the planctonic cells and the minimal biofilm eradication concentration(MBEC) of the cells in biofilm were undertaken.RESULTS P.aeruginosa biofilms were observed on the surface of catheters.The MBEC was 100-1000 times than MIC and MBC.CONCLUSION The assay of establishing P.aeruginosa biofilm and observed by silver staining is convenient and feasible.P.aeruginosa in biofilm exhibits far more resistance to antimicrobial than its planctonic counterparts does.