Anti-proliferation and inducing apoptosis effects of CIK cells on lung cancer cell lines

Purpose To study the anti-proliferation and inducing apoptosis effects of cytokine-induced killer cells-CIK cells on A549 lung cancer cell lines.Methods The anti-proliferation and the cytotoxicity of CIK cells on A549 lung cancer line were detected by MTT assay.The morphological changes of the apoptosis cell were observed by invert microscope,HE stain,transmission electron microscope and AO/EB double fluorescent staining.Results MTT assay showed that the inhibitory rate enhanced obviously with the addition of effect/target rate and extension of time(P0.01).Lung cancer cell apoptosis or necrosis were morphologically observed after lung cancer cells were induced by CIK cells for 24 hours.Conclusion CIK cells are highly effective cells which have a stronger anti-proliferation and inducing apoptosis effects on lung cancer cells.