Effect of fenofibrate on hepatocyte apoptosis and hepatic glycogen in rats with obstructive jaundice

Objective To investigate the effect of Fenofibrate on serum biochemical indicators,apoptosis and hepatic glycogen in rats with obstructive jaundice.Methods Rats were subjected to bile duct ligation to establish the cholestasis model.Forty-eight male Wistar rats were randomly divided into four groups equally: Group A = control group,Group B = bile duct ligation(BDL),Group C = BDL + Fenofibrate(30 mg/kg daily).Group D = BDL + Fenofibrate(60 mg/kg daily).All rats were sacrificed on 7th day after obtaining blood samples and liver tissue.Serum ALT,AST,ALP,GGT,TBA were tested by automatic chemistry analyzer,the number of hepatocyte apoptosis were tested by TUNEL and hepatic glycogen were tested by PAS.Results Serum ALT,AST,ALP,GGT,TBA levels in rats with BDL were significantly higher than in the group A(P0.001).There was no significant difference in terms of serum ALT,AST,ALP,GGT,TBA levels between group B and group C.Serum ALT,AST,ALP,GGT,TBA levels in rats of group D were significantly lower than in group B,C(P0.01) except ALT between Group D and Group C(P=0.0210.01).Hepatocyte apoptosis in rats with BDL were significantly higher than in the control group(P0.001).Hepatocyte apoptosis in the animals of group C were significantly lower than in group B(P=0.0070.01) when hepatocyte apoptosis in group D were significantly lower than in group B or C(P=0.0000.001).Periodic acid schiff(PAS) reaction indicated that fenofibrate can strengthen hepatic glycogen synthesis in rats with obstructive jaundice.Conclusion Fenofibrate administration attenuates serum biochemical indicators、hepatocyte apoptosis and strengthen hepatic glycogen synthesis in rats with obstructive jaundice.