The effect of Baicalein on the proliferation and cell cycle of EC109

Objective To investigate the effects and possible mechanisms of Baicalein on the proliferation and cell cycles of human esophageal carcinoma cell line EC109.Methods EC109 cells were treated with different BAI concentration(0,5,10,20,40 and 80(mol/L).Thiazolyl blue terazolium bromide(MTT) method and flow cytometry(FCM) were used to measure cell proliferation and cell cycle of EC109 cells,respetively.Results With the concentration from 0 to 80(mol/L and exposure time form 24 to 72 hours,BAI inhibited the proliferation of EC109 cells in concentration-dependent and time-dependent manners.Rating by concentration from 0 to 80(mol/L,the survival rates of cells were 98.4%,87.7%,87.6%,81.9% and 79.0%,respectively,in 24 h,71.6%,67.6%,64.1%,60.0% and 46.7%,respectively,in 48h and 43.8%,43.1%,43.1%,40.0%,23.0%,respectively,in 72 h.Cell cycle analysis revealed a marked increase from 43.6% to 68.5% in the G1 phase and a decrease from 24.5% to 0.0% in the G2 phase following 24 h exposure to BAI in a concentration-dependent manner.Conclusion BAI inhibits the proliferation of EC109 cells in concentration-dependent and time-dependent manners.BAI may change the cell cycles in a concentration-dependent manner.