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2010•Zhongguo linchuang yaolixue yu zhiliaoxueRequires access

Comparison of the inhibitory effect on HBV replication by siRNA and lamivudine in HepG2.2.15 cells

Lu Juan

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Abstract

AIM:To compare the inhibitory effect of inhibition of siRNA and lamivudine on hepatitis B virus replication and expression in HepG2.2.15 cells. METHODS: Recombinant plasmid psil-HBV was constructed and transfected into HepG2.2.15 cells. The transfected cells were harvested at 48,72 and 96 h. The concentration of HBeAg and HBsAg was determined using ELISA method. HBV DNA replication was examined by real time PCR, and the level of HBV mRNA was measured by reserved transcribed PCR.RESULTS:siRNA effectively inhibited the expressing and replication of HBV at the levels of HBsAg, HBV DNA and mRNA(P0.05). Lamivudine had only inhibitory effect on viral DNA synthesis. CONCLUSION: The anti-HBV effect of HepG2.2.15 cells treated by siRNA is totally differently and more effective from that seen with lamivudine in HepG2.2.15 cells.

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AIM:To compare the inhibitory effect of inhibition of siRNA and lamivudine on hepatitis B virus replication and expression in HepG2.2.15 cells. METHODS: Recombinant plasmid psil-HBV was constructed and transfected into HepG2.2.15 cells. The transfected cells were harvested at 48,72 and 96 h. The concentration of HBeAg and HBsAg was determined using ELISA method. HBV DNA replication was examined by real time PCR, and the level of HBV mRNA was measured by reserved transcribed PCR.RESULTS:siRNA effectively inhibited the expressing and replication of HBV at the levels of HBsAg, HBV DNA and mRNA(P0.05). Lamivudine had only inhibitory effect on viral DNA synthesis. CONCLUSION: The anti-HBV effect of HepG2.2.15 cells treated by siRNA is totally differently and more effective from that seen with lamivudine in HepG2.2.15 cells.

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Available abstract

AIM:To compare the inhibitory effect of inhibition of siRNA and lamivudine on hepatitis B virus replication and expression in HepG2.2.15 cells. METHODS: Recombinant plasmid psil-HBV was constructed and transfected into HepG2.2.15 cells. The transfected cells were harvested at 48,72 and 96 h. The concentration of HBeAg and HBsAg was determined using ELISA method. HBV DNA replication was examined by real time PCR, and the level of HBV mRNA was measured by reserved transcribed PCR.RESULTS:siRNA effectively inhibited the expressing and replication of HBV at the levels of HBsAg, HBV DNA and mRNA(P0.05). Lamivudine had only inhibitory effect on viral DNA synthesis. CONCLUSION: The anti-HBV effect of HepG2.2.15 cells treated by siRNA is totally differently and more effective from that seen with lamivudine in HepG2.2.15 cells.

Key concepts: Lamivudine, HBeAg, HBsAg, Transfection, Hepatitis B virus, Virology, Molecular biology, Recombinant DNA

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