DNA gel electrophoretic and microaut oradiographic studies on apoptosis in bone tumor cells after exposure with 153Sm-EDTMP

The apoptosis in bone tumor cells is studied after 153Sm-EDTMP irradiation. Fragmented DNA is analyzed by agarose gel electrophoresis. Experimental observations show that 153Sm-EDTMP exposure induces the internucleosomal DNA damage in bone tumor cells the DNA ladder pattern formation in bone tumor cells is shown. At the same time, the microautoradiographic study indicates that 153Sm-EDTMP could permeate through cell membrane and displays membrane-seeking condensation in bone tumor cells. Soon afterwards 153Sm-EDTMP could be phagocytized by the tumor cells and distributed ill cytoplasm as well as nucleus in the form of phagosome. With the prolongation of observing time, the membrane-bounded apoptotic bodies are observed.