Study on Genetic Diversity of Atremisia frigida by RAPD

Objective To study the genetic diversity of Atremisia frigida in Tongliao population and three different regions of Inner Mongolia(Tongliao,Hohhot,Xilingol).Methods 13 groups of primers with clear,obviously polymorphic and reproducible bands were screened from the 17 groups of primers by random amplified polymorphic DNA(RAPD)method.The bands produced by the 13 groups of primers were calculated and analyzed through Popgen32 and NTSYS software.Results ① 13 groups of primers generated 209 bands,including 201 polymorphic bands.The percentage of polymorphic loci was up to 96.17%.The Nei's genetic diversity and Shannon's information were different in the three different regions.② Total genetic diversity(Ht)of three Atremisia frigida population was 0.238 1.Genetic diversity within three populations(Hs)was 0.187 4.Genetic diversity among populations(Dst)was 0.050 7.Genetic differentiation coefficient among populations(Gst)was 0.212 9.③ In accordance with UPGMA based on genetic distance analysis,A.frigida of Hohhot and Xilingol clustered into one category,A.frigida of Tongliao clustered into a separate category.Conclusion ① A.frigida has a high genetic diversity,and the genetic diversities of A.frigida in three regions are different.② The total genetic diversity is from the genetic variation within A.frigida populations(78.71%).Meanwhile,there are genetic exchanges among populations.