Flow Injection Post-Chemiluminescence Analysis of Amikacin

In basic medium of pH=11.5,peroxymonosulfate(PMS),a strong oxidant,can oxidize the luminol to produce chemiluminescence,and after the injection of amikacin,the luminescence rate becomes slow and strong post-chemiluminescence(PCL).Based on this,a new CL method was developed for the determination of amikacin using flow injection chemiluminescence analysis.A possible mechanism for the CL was discussed via the studies of kinetic characteristics,the CL spectra and UV spectrum.The linear range was 4.0×10-5-8.0×10-2 g/L with a detection limit of 1×10-5 g/L for amikacin and the relative standard deviation was 2.9%(c=4.0×10-6 g/mL,n=11).The proposed method was selective,simple,rapid and reproducible,and has been successfully applied to the analysis of human urine samples with satisfactory results.