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2004•Journal of Southwest China Normal UniversityRequires access

Fading Reactions of Methyl Blue by Kanamycin, Neomycin and Their Analytical Applications

Zhan Hai-lin, HU Xiao-li

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Abstract

In the condition of pH 3.0-5.0, methyl blue (MB) reacts with kanamycin sulfate (KANA) or neomycin sulfate (NEO) to form ion association complexes, which lead to SE fading. The maximum fading wavelengths were located 608 nm(KANA) and 606 nm (NEO); The apparent molar absorptivity (e) is 2.44×10~4 L·mol~(-1)·cm~(-1) for KANA and 2.99×10~4 L· mol~(-1)·cm~(-1) for NEO; Beer' law is obeyed in definite concentration arange. The method was applied to determine the content of the real sample with satisfactory results.

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What this paper is about

In the condition of pH 3.0-5.0, methyl blue (MB) reacts with kanamycin sulfate (KANA) or neomycin sulfate (NEO) to form ion association complexes, which lead to SE fading. The maximum fading wavelengths were located 608 nm(KANA) and 606 nm (NEO); The apparent molar absorptivity (e) is 2.44×10~4 L·mol~(-1)·cm~(-1) for KANA and 2.99×10~4 L· mol~(-1)·cm~(-1) for NEO; Beer' law is obeyed in definite concentration arange. The method was applied to determine the content of the real sample with satisfactory results.

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Available abstract

In the condition of pH 3.0-5.0, methyl blue (MB) reacts with kanamycin sulfate (KANA) or neomycin sulfate (NEO) to form ion association complexes, which lead to SE fading. The maximum fading wavelengths were located 608 nm(KANA) and 606 nm (NEO); The apparent molar absorptivity (e) is 2.44×10~4 L·mol~(-1)·cm~(-1) for KANA and 2.99×10~4 L· mol~(-1)·cm~(-1) for NEO; Beer' law is obeyed in definite concentration arange. The method was applied to determine the content of the real sample with satisfactory results.

Key concepts: Fading, Kanamycin, Resorcinol, Molar absorptivity, Neomycin, Chemistry, Kana, Analytical Chemistry (journal)

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