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2006•Xi'nan Nongye Daxue xuebaoRequires access

EFFECTS OF SEVERAL FACTORS ON CALLUS INDUCTION FROM SEEDS OF POA PRATENSIS L.

Pi Wei

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Abstract

Seeds of Poa pratensis L.were cultured under 12 h L + 12 h D photoperiod in different seasons in MS medium supplemented with different sucrose concentrations to induce callus differentiation.Culture in the dark gave higher induction rate(55.6%) than 12 h L + 12 h D photoperiod(38.1%).A sucrose concentration of 30 or 15 g/L gave satisfactory results,the callus induction rate being(62.5%) and 58.3%,respectively.Culture in spring,summer or autumn was suitable while culture in winter was not.

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Seeds of Poa pratensis L.were cultured under 12 h L + 12 h D photoperiod in different seasons in MS medium supplemented with different sucrose concentrations to induce callus differentiation.Culture in the dark gave higher induction rate(55.6%) than 12 h L + 12 h D photoperiod(38.1%).A sucrose concentration of 30 or 15 g/L gave satisfactory results,the callus induction rate being(62.5%) and 58.3%,respectively.Culture in spring,summer or autumn was suitable while culture in winter was not.

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Available abstract

Seeds of Poa pratensis L.were cultured under 12 h L + 12 h D photoperiod in different seasons in MS medium supplemented with different sucrose concentrations to induce callus differentiation.Culture in the dark gave higher induction rate(55.6%) than 12 h L + 12 h D photoperiod(38.1%).A sucrose concentration of 30 or 15 g/L gave satisfactory results,the callus induction rate being(62.5%) and 58.3%,respectively.Culture in spring,summer or autumn was suitable while culture in winter was not.

Key concepts: Callus, Poa pratensis, photoperiodism, Sucrose, Botany, Biology, Horticulture, Poaceae

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EFFECTS OF SEVERAL FACTORS ON CALLUS INDUCTION FROM SEEDS OF POA PRATENSIS L. — Research Paper | ScholarLens