Synergistic induction of apoptosis in HBV expressing HepG2.2.15 cells by interferon gamma and tumor necrosis factor alpha

Purpose The aims of this study were to examine the cell kinetics and apoptosis in IFNγ and TNF-α treated HepG2.2.15 cells and its meanings. Methods Cell morphologic feature was observed by light microscopy.Cell viability was assessed by MTT assay quantitatively.The typical DNA ladder test was made with agarose gel electrophoresis in order to see whether the changes of cell morphology and the reduction of cell viability are due to apoptosis. Results 1 000 U/mL IFN-γ alone or combined with 5 ng/mL TNF-α induced apoptosis in HepG2.2.15 cells.Lamivudine treatment could reduce IFN-γ and TNF-α mediated apoptosis in HepG2.2.15 cells. Conclusions Above results demonstrated that IFN-γ and TNF-α induced apoptosis in HBV expressing HepG2.2.15 cells synergistically.Inhibition of HBV replication by Lamivudine treatment of HepG2.2.15 cells reduced IFN-γ + TNF-α-induced apoptosis.