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Effect of TNF-α on the apoptosis of A549 cells

Jianqi Yu

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Abstract

AIM: To observe the effect of TNF-α on the apoptosis of A549 cells. METHODS: A549 cells were cultured and incubated with TNF-α (10, 100 U·ml -1 ). Western blot was used to observe the change of P53 protein, and the cell apoptosis was observed by the Annexin V/PI staining. RESULTS: The expression of P53 protein and the number of apoptotic cells increased significantly after treatment with TNF-α as compared with the control group (P 0.01 ). The apoptosis rate of A549 cells was 1.22 ± 0.62 and 1.65 ± 0.38 in the TNF-α group with different dose respectively (P 0.01 ). CONCLUSIONS: TNF-α can induce the apoptosis of A549 cells and maybe provide the new idea for the therapy of lung cancer.

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What this paper is about

AIM: To observe the effect of TNF-α on the apoptosis of A549 cells. METHODS: A549 cells were cultured and incubated with TNF-α (10, 100 U·ml -1 ). Western blot was used to observe the change of P53 protein, and the cell apoptosis was observed by the Annexin V/PI staining. RESULTS: The expression of P53 protein and the number of apoptotic cells increased significantly after treatment with TNF-α as compared with the control group (P 0.01 ). The apoptosis rate of A549 cells was 1.22 ± 0.62 and 1.65 ± 0.38 in the TNF-α group with different dose respectively (P 0.01 ). CONCLUSIONS: TNF-α can induce the apoptosis of A549 cells and maybe provide the new idea for the therapy of lung cancer.

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Available abstract

AIM: To observe the effect of TNF-α on the apoptosis of A549 cells. METHODS: A549 cells were cultured and incubated with TNF-α (10, 100 U·ml -1 ). Western blot was used to observe the change of P53 protein, and the cell apoptosis was observed by the Annexin V/PI staining. RESULTS: The expression of P53 protein and the number of apoptotic cells increased significantly after treatment with TNF-α as compared with the control group (P 0.01 ). The apoptosis rate of A549 cells was 1.22 ± 0.62 and 1.65 ± 0.38 in the TNF-α group with different dose respectively (P 0.01 ). CONCLUSIONS: TNF-α can induce the apoptosis of A549 cells and maybe provide the new idea for the therapy of lung cancer.

Key concepts: Apoptosis, Annexin, A549 cell, Tumor necrosis factor alpha, Western blot, Molecular biology, Chemistry, Blot

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