Study of extracting water-soluble polysaccharide from dehulled mung bean by response surface methodology

Experiments concerning extracting polysaccharide from dehulled mung bean have been conducted.Particularly,the effects of factors( extraction time,the ration of raw materials to water,extraction times and extraction temperature) on the yield of polysaccharide had been studied as well. Based on the single- factor experiments,response surface methodology had been employed to optimize extraction conditions. Results indicated that the optimum conditions for polysaccharide from dehulled mung bean extraction were: water temperature of 95.5℃,a ratio of water to raw materials of 21 ∶ 1( V/m) and an extraction time of 3h. Under this conditions,the yield of polysaccharide reached 4.32%. Following this,Sevag methodology had been used to remove the protein contained in polysaccharide as well.After deproteination,the content of polsaccharide reached50.35% while there was still approximately 31.26% of protein.