The effects of IL-23 alone or combined with IL-2 on the proliferation and antileukemia activity of PBMC

We aim to investigate the effects of IL-23 alone or combined with IL-2 on the proliferation and anti-leukemia activity of human peripheral blood mononuclear cells(PBMC).PBMCs were separated by ficoll density gradient centrifugation,and the proliferation and cytotoxic activity against K562 were investigated by MTT assay.Changes in cell phenotype of PBMC were detected by Flow cytometry.PBMC's proliferation and cytotoxic activity against K562 cells was enhanced in experiment groups after treatment for 1,3,5 days,which could be synergized by IL-23 plus IL-2 had.Surface markers of PBMC were significantly increased(P 0.05) after treatment with IL-23(50 ng/ml) or IL-23(50 ng/ml) +IL-2(100 IU/ml) for 5 days: CD3+ cells were(80.7±4.37)% and(83.2±4.04)%,CD16+CD56+ cells were(8.4±0.28)% and(12.7±0.9)%,CD4+ cells were(45.2±1.4)% and(47.0±1.72)%,CD8+ cells were(34.5±2.53)% and(35.4±2.11)%.The expression of CD16+CD56+ cells demonstrated significant difference between the two groups(P 0.05),but there was no significant difference of the expression of CD3+ cells,CD4+ cells,and CD8+ cells between the two groups(P 0.05).The result suggested that IL-23 could enhance PBMC's proliferation and cytotoxic activity,especially combined with IL-2.Furthermore,L-23 alone or combined with IL-2 could increase surface markers of PBMC,and IL-23 combined with IL-2 can further enhance the antigen expression of CD16/56.