IL-2,IL-21 induced human peripheral blood mononuclear cells and its anti-tumor effects

Objective To investigate the changes of proliferation,phenotype and in vitro antitumor activity of human peripheral blood mononuclear cells(PBMC) induced by IL-2 and IL-21.Methods Sort PBMCs into 4 gropus in the concentration of 1×106 /ml,IL-2 of 100 IU/ml was added into IL-2 group,IL-21 of 100 IU/ml was added into IL-21 group,IL-2 of 50 IU/ml and IL-21 of 50 IU/ml was added into joint group.Saline solution of 0.1 ml was added into the control group.Flow cytometry assay was used to analysis the subsets of PBMC after being induced and Trypan blue staining of living cells was counted in each group.The above groups was taken as effector cells(E),the logarithmic phase of 4 kinds of tumor cells(human gastric cancer cell line M85,gastric cancer cell lines BGC823,colon cancer cell line HCT116,colorectal adenocarcinoma cell line HCT8) as target cells(T),dilute targets cells into 5×103 cells/well,the ratio of E∶T was 1∶1 and 2∶1.MTT assay was used to detect the cytotoxic effects(killing ratio).Results Live cell counts in the joint group was higher than that tin the IL-2 group,IL-21 group and control group,all P0.05;CD3-/CD56+,CD3+/CD56+ in the joint group,IL-2 group and IL-21 group were higher than those in the control group,P0.05 or 0.01.The killing ratio of 4 kinds of tumor cells in the joint group was higher than the other three groups,and which in IL-2 group,IL-21 group was higher than that in the control group,P0.05.Conclusion IL-2 combined synergistic stimulation of IL-21 can effectively stimulate PBMC proliferation and phenotypic changes,and can kill 4 kinds of digestive tract cancer cell lines strongly.