Effects of Mercury Stress on Wheat Growth,POD and Amylase Activity
Lin Wang
Abstract
Lin Wang
Abstract
[Objective] The aim was to study the effect of mercury stress on seed germination and seedlings growth.[Method] Zhengzhou 9023 was used as the experimental material and cultured in water.After the seeds were soaked in different concentrations of Hg2+(0.025,0.050,0.100,0.200,0.300,0.400 and 0.500 mmol/L),the germination rate and seedling height,root length,seedling's fresh weight,and the activities of peroxidase(POD) and amylase in leaf,root and germinating embryo were measured.[Result] Low concentrations of Hg2+(≤0.100 mmol/L) had little effect on seed germination,seedling height,root length and fresh weight;high concentrations of Hg2+(0.10 mmol/L) could significantly inhibit seed germination and seedling growth.Low concentration of Hg2+(≥ 0.025 mmol/L) could significantly increase POD activity and inhibit the amylase activity,and the effects increased with the increasing of Hg2+concentrations.[Conclusion] Hg2+ stress could change the activities of POD and amylase in leaf,root and germinating embryo,thus influence the energy and substrate supply which was required for normal metabolism of lipid oxidation,and inhibit seedling growth ultimately.
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[Objective] The aim was to study the effect of mercury stress on seed germination and seedlings growth.[Method] Zhengzhou 9023 was used as the experimental material and cultured in water.After the seeds were soaked in different concentrations of Hg2+(0.025,0.050,0.100,0.200,0.300,0.400 and 0.500 mmol/L),the germination rate and seedling height,root length,seedling's fresh weight,and the activities of peroxidase(POD) and amylase in leaf,root and germinating embryo were measured.[Result] Low concentrations of Hg2+(≤0.100 mmol/L) had little effect on seed germination,seedling height,root length and fresh weight;high concentrations of Hg2+(0.10 mmol/L) could significantly inhibit seed germination and seedling growth.Low concentration of Hg2+(≥ 0.025 mmol/L) could significantly increase POD activity and inhibit the amylase activity,and the effects increased with the increasing of Hg2+concentrations.[Conclusion] Hg2+ stress could change the activities of POD and amylase in leaf,root and germinating embryo,thus influence the energy and substrate supply which was required for normal metabolism of lipid oxidation,and inhibit seedling growth ultimately.
Key concepts: Seedling, Germination, Point of delivery, Amylase, Peroxidase, Horticulture, Embryo, Biology