Study on Qualtaive Method of Salidroside of Artifical Cultivation Ultivation Rhodiola Rosea by HPLC-MS

To determine the petals,stems,roots planted content Rhodiola different medicinal parts。 Method: Each 1 g sample is dissolved after 60% ethanol,ultrasonic cleaner cleaning 50 min,filtered,rotary evaporated,add 2 mL of methanol,microporous filter membrane( 0. 45 μm) filter using CAPCELL PAK C18 column(( 2. 0 mm × 150 mm,i. d.,3 μm),column temperature 30 ℃,wavelength 278 nm,sample temperature 10 ℃,injection volume of 10 μL. The mobile phase was methanol-1% acetic acid solution 45 ∶55, flow rate of 1 mL·min- 1for quantitative analysis. Result: The effective parts of salidroside content in the order of the petals( 35. 00 mg),root( 18. 89 mg),stems( 1. 59 mg). Conclusion: A detection of salidroside content in Rhodiola rosea was established by high performance liquid chromatography. It can be applied to the analysis on the salidroside in the different medicinal materials.