Supercritical Carbon Dioxide Extraction of Salidroside from Rhodiola rosea L var. rosea Root

Abstract Salidroside from the root of Rhodiola rosea L var. rosea was extracted by supercritical carbon dioxide with and without methanol as modifier. Three parameters, i.e. temperature, pressure and different concentrations of methanol were optimized. Salidroside determinations were carried out using high‐performance liquid chromatography (HPLC) with UV‐Vis detector. An experimental design of response surface methodology (RSM) was used to map the effect of pressure (at 200, 300 and 400 bar), temperature (at 50, 60 and 70 °C) and percentage of methanol modifier (at 80, 90 and 100%) on the extraction yield of the active compound and to determine the optimal conditions for the extraction of salidroside from the root of plant. The results showed that supercritical carbon dioxide failed to extract salidroside from the plant material without a methanol as modifier. The yield obtained after 1.5 h extraction with the rate of modifier 0.4 mL/min and 300 bar, 70 °C, and 80 percent of methanol modifier condition was the highest (17.15 mg/g). The optimum conditions were 70 °C, 295.49 bar and 80 percent of methanol as modifier with the yield of 16.17 mg/g. In addition, the yield obtained with supercritical fluid extraction (SFE) was compared with the Soxhlet extraction, whose yield was 8.64 mg/g.