Study on Localization of Interleukin 8 in Mouse Endometrium and Embryo before and after Implantation

Objective: To study the localization of interleukin-8(IL-8) in mouse endometrium and embryo before and after implantation. Metheds: The local expressions of IL-8 in the endometrium and embryo of pregnant mouse were observed by immunohistochemistry method on the fourth and eighth day respectively. Results: On the fourth day,IL-8 was located chiefly in the luminal epithelium、glandular epithelium and stroma of endometrium, and positive IL-8 staining of blastocyst was showed in the inner cell clusters and trophoblast of blastocyst. On the eighth day, the expression of IL-8 was negative in luminal epithelium of endometrium, but the positive stainings in glandular epithelium and decidual cells were weak. However no staining was observed in embryo. Conclusions: These results indicated that IL-8 maybe participate in the process of mouse blastocyst implantation, but it had no relation to embryonic differentiation and embryogenesis.