Study of expression of P53、PCNA protein and cell apoptosis in gastric carcinoma

Objective To investigate the expression of P53, PCNA protein and cell apoptosis in gastric carcinoma.Methods Flow cytometry and TUNEL were used to assay cell apoptosis and expression of P53, PCNA protein in 60 cases of gastric carcinoma.Results The ratio of apoptosis was significantly lower than that in paracancer tissue( 0.01), and the expression of P53, PCNA protein were much higher than those in paracancer tissue.The cell apoptosis was lower and fluorescent intensity was higher in the group of positive expression of P53.The expression of P53 and PCNA in low-differentiated gastric cancer were higher than those in well-differentiated type.The expression of P53 and PCNA in gastric cancer without lymph node metastasis was lower than those with lymph node metastasis.Conclusion Apoptosis is significantly inhibited in gastric carcinoma, and increased expression of P53 protein and proliferation may play an important role in the progression of gastric carcinoma.