Experimental Research on Variation of Uterine Tension and Temperature of the Surface Area of Dysmenorrhea Rats with Cold Stagnation Syndrome and the Effect of Moxibustion Intervention

Objective: To observe the effect ofdifferent intervention time ofmoxibustion on bilateral Sanyinjiao( SP6) and Line Sanyinjiao( SP6)- Xuehai( SP10) of dysmenorrhea rats with the cold stagnation syndrome and its correlation with analgesic effect. Methods: Through the vaginal smear method,screen out thediestrus 3 monthsfemale SD rats. Rats during diestrus were randomized into a saline control group,a model control group and a moxibustion Sanyinjiao 1 time group( moxibustion on the 10 th day),a moxibustion Sanyinjiao 3 times group( moxibustion on the Sixth,the eighth andthe10 th day),each group with 20 rats. Except the saline control group,other groups were modeled with the whole body freezing method combined withthe method ofestradiol benzoate injection. Use infrared thermal imager to detect the temperature around Sanyinjiao( SP6) and Line Sanyinjiao( SP6)- Xuehai( SP10) area at each point of time. Use BL-420 E plus biological and functional experimental system to detect the intensity of uterine contraction. Results: Compared with the saline group,the model group significantly increased the intensity of uterine contractions( P 0. 01); compared with the model group,the two moxibustion groups significantly reduced the number of uterine contraction waves( P 0. 01),and reduced uterine activity( P 0. 01). Therewere not statistically significant differences between the twomoxibustion groups( P 0. 05); temperature of ratson left and right sides of the Sanyinjiao( Sp6) and Line Sanyinjiao( SP6)- Xuehai( SP10) area in saline control group was balanced at each point of time( P 0. 05). While temperature of rats on left and right sides of Sanyinjiao in the model control group was imbalanced at 5- 60 points( 5,40,50,60 P 0. 05,10,20,30 P 0. 01),andtemperature of Line Sanyinjiao( SP6)- Xuehai( SP10) area was the same at immediate- 30points( 5,30 P 0. 05,immediate,10,20 P 0. 01). Temperature of two moxibustiongroups on leftand right sides of Sanyinjiao( SP6) and Line Sanyinjiao( SP6)- Xuehai( SP10) area was balanced( P 0. 05). Conclusion: Moxibustion Sanyinjiao can significantly relieve the contraction of dysmenorrhea rats' uterus with cold stagnation syndrome and improve the imbalance oftemperature on left and right sides of Sanyinjiao( SP6) and Line Sanyinjiao( SP6)- Xuehai( SP10) area.Different intervention time ofmoxibustionmakes no difference forrelieving the pain of dysmenorrhea.