Effect of intranasal pGEG.mIL-12/lipoplex on the eosinophils in the murine model of allergic rhinitis

OBJECTIVE To evaluate the effect of intranasal liposome-mediated IL-12 gene therapy on the eosinophils in the murine model of allergic rhinitis. METHODS Thirty-six BALB/C mice were randomly divided into allergic rhinitis(AR)group, gene therapy group and control group. Allergic rhinitis group were sensitized and suscitated ovalb umin(ovalbumin, OVA), and gene therapy group were administered with liposome-mediated pGEG.mIL-12 per nasal before suscitation. The eosinophils in bone marrow were counted by Wright's staining, and the eosinophils in nasal mucosa were counted by H-E staining. The eosinophils of peripheral blood were detected by flow cytometry. RESULTS The ratio of eosinophils to white cells in nasal mucosa and bone marrow of gene therapy group was significantly lower than that of AR group(P0.01).The ratio of eosinophils to granulocyte in peripheral blood were significantly lower in gene therapy group than in AR group(P 0.05). CONCLUSION Administration of liposomemediated pGEG.mIL-12 per nasal could depress the proliferation and differentiation of eosinophils and decrease the delivery and transference of eosinophils to peripheral blood and nasal mucosa. It probably develops a new treatment for respiratory tract allergic inflammation.