Observation on the Nasal Mucosa Remodeling in Mouse Model of Allergic Rhinitis

Objective: To observe the nasal tissue remodeling in a mouse model of allergic rhinitis. Methods: Twenty BALB/c mice were randomly divided into the allergic rhinitis group and control group. Ovalbumin(OVA) was used to establish the mouse allergic rhinitis model. Hematoxylin-eosin(HE) staining, Giemsa staining and Alcianblue-periodicacid-Schiff staining were carried out to observe the tissue remodeling, eosinophils and goblet cells, respectively. Enzyme linked immunosorbent assay(ELISA) was used to detect the serum interleukin 4(IL-4) level. Results: The mouse allergic rhinitis model was successfully established, the score of biological behaviour was(6.5±1.3). Structural remodeling occured in nasal mucosa of allergic rhinitis group. Compared with the control group,there were obviously cell desquamation, necrosis, squamous metaplasia and goblet cell hyperplasia in epithelial layer. In the lamina propria and submucosa, glandular hyperplasia, vasodilatation, edema and characteristic eosinophils infiltration were also found in the allergic rhinitis group. The eosinophils and goblet cell counts were 26.4±5.72 and 24.14±3.12 in allergic rhinitis group respectively,which were 8.31±2.42 and 9.41±1.22 in the control group respectively(P0.05). The serum IL-4 level was(18.9±3.1)pg/ml in allergic rhinitis group and(8.3±1.4)pg/ml in control group, which was significantly higher in allergic rhinitis group than that of the control group(P0.05). Conclusion: Structural remodeling occured in the nasal mucosa of allergic rhinitis mouse induced by OVA.