Study of Protective Effects of Magnesium Isoglycyrrhizinate on Liver Injury Caused by Biliary Obstruction in Rat

Objective: To investigate the protective effect of Magnesium Isoglycyrrhizinate in rat liver injury of obstructive jaundice. Methods: Thirty-two healthy male SD rats were randomly divided into 4 groups(8 rats in each group),including Bile duct ligation and conventional dose of Magnesium Isoglycyrrhizinate injection group(BMc group),Bile duct ligation and high dose of Magnesium Isoglycyrrhizinate injection group(BMh group),Bile duct ligation and 0.9% Normal saline saline injection group(BN group)and sham-operation group(Sham group). The rats from BN group and Sham group were intraperitoneal injected normal saline by 30mg/kg/day,while the rats from BMc group and BMh group were intraperitoneal injected Magnesium Isoglycyrrhizinate by 30 mg/kg/day and 60mg/kg/day respectively. The blood were collected from orbital cavity and centrifuged to obtain serum. The liver function indexes were analyzed by automatic biochemical instrument,including Alanine aminotransferase(ALT),Aspartate Aminotransferase(AST) and Direct Bilirubin(DBil). Results: The ALS and AST levels significantly increased in rat serum from BN,BMc and BMh groups after bile duct ligation. Along with the increase of the obstruction time,the ALT and AST levels in rat serum from BMc and BMh group were significantly lower than those in BN group(P0.01),but no significant difference was found between these two groups. The serum levels of D-Bil from BN,BMc and BMh groups rats were apparently higher than that in Sham group(P0.01),but there were no difference among the three groups along with the increase of the obstruction time. Conclusion: Magnesium Isoglycyrrhizinate could effectively reduce the serum transaminase levels stimulated by obstructive jaundice,and play important protective roles in liver function.