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2014•Zhongguo laonianxue zazhiRequires access

Effect of Jinxiangdan on apoptosis in rats with myocardial ischemia-reperfusion injury

Chao Wang

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Abstract

Objective To explore study the effects of Jinxiangdan on the apoptosis in rats with myocardial ischemia-reperfusion injury. Methods Rats models with myocardial ischemia-reperfusion injury were established by occluding left coronary artery( LCA) and followed by release of it with ligation. TUNEL and S-P immunohistochemistry staining were used to determine the apoptosis and protein expression of Bax,Bcl-2 and Caspase-3 genes. Results The protein expressions of Bax,Bcl-2 and Caspase-3 were increased in the ischemiareperfusion group. The protein expressions of Bax and Caspase-3 were decreased,Bcl-2 was increased significantly in the Jinxiangdan group, compared with those of the ischemia-reperfusion group. Conclusions Jinxiangdan has a good antagonist action on the apoptosis of ischemia / reperfusion myocardial cells,and its mechanisms maybe by increasing the expression of Bcl-2 as well as reducing the expression of Bax and Caspase-3.

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What this paper is about

Objective To explore study the effects of Jinxiangdan on the apoptosis in rats with myocardial ischemia-reperfusion injury. Methods Rats models with myocardial ischemia-reperfusion injury were established by occluding left coronary artery( LCA) and followed by release of it with ligation. TUNEL and S-P immunohistochemistry staining were used to determine the apoptosis and protein expression of Bax,Bcl-2 and Caspase-3 genes. Results The protein expressions of Bax,Bcl-2 and Caspase-3 were increased in the ischemiareperfusion group. The protein expressions of Bax and Caspase-3 were decreased,Bcl-2 was increased significantly in the Jinxiangdan group, compared with those of the ischemia-reperfusion group. Conclusions Jinxiangdan has a good antagonist action on the apoptosis of ischemia / reperfusion myocardial cells,and its mechanisms maybe by increasing the expression of Bcl-2 as well as reducing the expression of Bax and Caspase-3.

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Available abstract

Objective To explore study the effects of Jinxiangdan on the apoptosis in rats with myocardial ischemia-reperfusion injury. Methods Rats models with myocardial ischemia-reperfusion injury were established by occluding left coronary artery( LCA) and followed by release of it with ligation. TUNEL and S-P immunohistochemistry staining were used to determine the apoptosis and protein expression of Bax,Bcl-2 and Caspase-3 genes. Results The protein expressions of Bax,Bcl-2 and Caspase-3 were increased in the ischemiareperfusion group. The protein expressions of Bax and Caspase-3 were decreased,Bcl-2 was increased significantly in the Jinxiangdan group, compared with those of the ischemia-reperfusion group. Conclusions Jinxiangdan has a good antagonist action on the apoptosis of ischemia / reperfusion myocardial cells,and its mechanisms maybe by increasing the expression of Bcl-2 as well as reducing the expression of Bax and Caspase-3.

Key concepts: Apoptosis, TUNEL assay, Ischemia, Reperfusion injury, BAX Protein, Immunohistochemistry, Medicine, Ligation

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