2013•Chinese Journal of Otorhinolaryngology-skull Base SurgeryRequires access
Recombinant adenovirus transfects organ of Corti from neonatal mouse in vitro
Xia Gao
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Abstract
Objective To detect the transfection of organ of Corti from neonatal mouse with Ad-GFP in vitro.Methods One day after tissue cultivation,the organ of Corti from neonatal mouse was transfected with Ad-GFP in vitro.One day after transfection,the transfection efficiency was analyzed with Confocal and fluorescence microscopes.Results One day after the tissue cultivation,the organ of Corti grew wel1 and new epithelial cells and fibroblasts were found around the tissue.One day after the transfection,the hair cells,the cells in lesser and greater epithelial ridges,the new epithelial cells and fibroblasts around the tissue were effectively transfected by Ad-GFP.Conclusions Ad-GFP can transfect organ of Corti from neonatal mouse effectively in vitro.