pIRES2-EGFP-NT3 transfected cultivation of basal membrane of Corti's organ from neonatal mouse in vitro with Lipofectamine 2000

Objective To construct pIRES2-EGFP-NT3 and detect its transfection in the in vitro cultivation of the basal membrane of Corti's organ from neonatal mouse.Methods After successful construction of pIRES2-EGFP-NT3,the in vitro cultivation of Corti's organ from neonatal mouse was transfected by pIRES2-EGFP-NT3 with the mediation of Lipofectamine TM2000.The transfection efficiency was analysed under confocal microscope 24 hours later.Results The pIRES2-EGFP-NT3 was successfully constructed and properly transfected into 17 cells of the basal membrane of Corti's organ which was cultivated in vitro with the mediation of cationic liposome.Conclusion The successful construction of pIRES2-EGFP-NT3 and its efficient transfection into cultivation of Corti's organ from neonatal mouse by LipofectamineTM2000 lay the groundwork for the following experiments,such as NT3 gene transfection in deaf or nomal cochlea.