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2001•Chinese journal of integrated traditional and Western medicineRequires access

Effect of Ginkgo Biloba Extract (GbE) on Expression of TGF-β1mRNA and Deposititon of Collagen in CCl_4 Rats

Hengmei Shi

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Abstract

To investigate the effect of Ginko Biloba extract (GbE) on expression of TGF-β1 mRNA and deposition of collagen in CC14 rats. Methods: 40 Wistar rats were divided into 3 gronps: control group (n=10). CC14 grouop (n = 15),GbE group (n=15). Ry the end of 12th week, the rats were killed. Degrees of fibrosis were determined by histopathology under microscopy and electron microscopy; The expression of TGF-β1 mRNA was measured by reverse transcription polymerase chain reaction (RT-PCR) ; MDA in hepatic tissue and serum PAF were measured also. Results: In CC14 group 8 rats developed to cirrhosis, while in GbE group only 3 rats produced cirrhosis; Compared with CC14 group, the TGF-β1 mRNA level was remarkedly decreased(P0. 05)and the content of MDA and PAF were significiant-ly reduced in GbE group(P0. 05). Conclusion:GbE can resist PAF,scavenge free redical and downregulate the expression of TGF-β1 mRNA, and therefore inhibit deposition of collagen in hepatic tissue.

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What this paper is about

To investigate the effect of Ginko Biloba extract (GbE) on expression of TGF-β1 mRNA and deposition of collagen in CC14 rats. Methods: 40 Wistar rats were divided into 3 gronps: control group (n=10). CC14 grouop (n = 15),GbE group (n=15). Ry the end of 12th week, the rats were killed. Degrees of fibrosis were determined by histopathology under microscopy and electron microscopy; The expression of TGF-β1 mRNA was measured by reverse transcription polymerase chain reaction (RT-PCR) ; MDA in hepatic tissue and serum PAF were measured also. Results: In CC14 group 8 rats developed to cirrhosis, while in GbE group only 3 rats produced cirrhosis; Compared with CC14 group, the TGF-β1 mRNA level was remarkedly decreased(P0. 05)and the content of MDA and PAF were significiant-ly reduced in GbE group(P0. 05). Conclusion:GbE can resist PAF,scavenge free redical and downregulate the expression of TGF-β1 mRNA, and therefore inhibit deposition of collagen in hepatic tissue.

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Available abstract

To investigate the effect of Ginko Biloba extract (GbE) on expression of TGF-β1 mRNA and deposition of collagen in CC14 rats. Methods: 40 Wistar rats were divided into 3 gronps: control group (n=10). CC14 grouop (n = 15),GbE group (n=15). Ry the end of 12th week, the rats were killed. Degrees of fibrosis were determined by histopathology under microscopy and electron microscopy; The expression of TGF-β1 mRNA was measured by reverse transcription polymerase chain reaction (RT-PCR) ; MDA in hepatic tissue and serum PAF were measured also. Results: In CC14 group 8 rats developed to cirrhosis, while in GbE group only 3 rats produced cirrhosis; Compared with CC14 group, the TGF-β1 mRNA level was remarkedly decreased(P0. 05)and the content of MDA and PAF were significiant-ly reduced in GbE group(P0. 05). Conclusion:GbE can resist PAF,scavenge free redical and downregulate the expression of TGF-β1 mRNA, and therefore inhibit deposition of collagen in hepatic tissue.

Key concepts: Ginkgo biloba, Transforming growth factor, Chemistry, Messenger RNA, Cirrhosis, Reverse transcription polymerase chain reaction, Endocrinology, Histopathology

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