Anti-hepatic Fibrosis in Rats and It's Mechanism of Ginkgo Biloba Extrac GbE761

Objective To investigate the effect of Ginkgo biloba extract GbE761 on hepatic fibrosis in rats and its mechanism.Methods The rat model with hepatic fibrosis was induced by carbon tetrachloride(CCL4). GbE761 at the dose of 0.3 mg/kg,0.6 mg/kg,0.9 mg/kg had been given orally for 30 days.ALT,AST and MDA were determined by routine methods.Precollagen typeⅢ(PCⅢ),hyaluronate(HA),laminin(LN) were determined by radioimmunoassay.HSC-T6 were cultured with GbE761 in different concentrations(1,10,100,500mg/L) and took that without GbE761 as blank conrol.MTT colorimetric assay was used for detecting the proliferation of HSCs and flow cytometry was performed for observing the cell cycles of HSCs under different concentrations of GbE761.Results GbE761 could lower the serum HA,LN,PCⅢ,ALT,AST and the tissue MDA more significantly than the model group.The effect was increased with a dose dependent manner.It could inhibit the proliferation of HSCs with the cell cycle being blocked in G1 phase at the concentration of 10,100 and 500 mg/kg.Conclusion GbE761 can attenuate hepatic fibrosis induced by CCL4 and GbE761 can exert significant effect on HSC-T6 by inhibiting the proliferation.